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Kevin Madauss Phones & Addresses

  • Phoenixville, PA
  • Oaks, PA
  • 1317 Checkerberry Dr, Morrisville, NC 27560
  • Wade, NC
  • 1348 Catrush Way, Wake Forest, NC 27587
  • Apex, NC
  • Raleigh, NC

Publications

Us Patents

Mutations In The Mineralocorticoid Receptor Ligand Binding Domain Polypeptide That Permit Structural Determination Of Low Affinity Ligand Complexes And Screening Methods Employing Same

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US Patent:
2007021, Sep 20, 2007
Filed:
Mar 16, 2006
Appl. No.:
11/377502
Inventors:
Christopher Apolito - Durham NC,
Randy Bledsoe - Durham NC,
Millard Lambert - Durham NC,
Kevin Madauss - Durham NC,
Thomas Stanley - Durham NC,
Eugene Stewart - Durham NC,
Shawn Williams - Durham NC,
International Classification:
C07K 14/715
G06F 19/00
US Classification:
530350000, 702019000
Abstract:
An isolated mineralocorticoid receptor (MR) polypeptide, or functional portion thereof, having one or more mutations that alter the solubility or crystal-forming properties and confer the ability to generate soluble protein complexes with the MR and ligands that only weakly bind the native polypeptide, and a polynucleotide encoding it are disclosed. Representative mutations are C808S and S810L substitutions. Expression of the MR polypeptide in is also provided. A solved three-dimensional crystal structure of an MR ligand binding domain polypeptide is also disclosed, along with a crystalline form of the MR ligand binding domain polypeptide. Methods of modeling one or more molecular interactions of a native NR with a ligand having low affinity for the native NR utilizing a mutated MR, designing modulators of the biological activity of MR and other nuclear receptor, steroid receptor and glucocorticoid receptor polypeptides and nuclear receptor, steroid receptor and glucocorticoid receptor ligand binding domain polypeptides are also disclosed.
Kevin P Madauss from Phoenixville, PA, age ~49 Get Report